Multiplex and multicolor capabilities permit the use of up to four different fluorescent dyes. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. The lightcycler 96 system is an optimal midsize, midthroughput qpcr solution for academic research, providing an ideal combination of temperature homogeneity and data reproducibility. Mar 31, 2020 i am using lightcycler 96 for absolute quantification, and want to generate a standard curve, as i am using one reference gene with the 10 different samples with different cdna dilutions such. A cc object can only be applied to experiments that were. Compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis, mirna research, and relative quantification of target genes.
Lightcycler 96 novy lightcycler 96 system byl vyvinut na zaklade nasich dlouholetych zkusenosti s realtime pcr. Benefit from a large, intuitive touchscreen interface. Supported by a broad range of software tools, realtime pcr based analysis can be performed in 32 capillaries, interchangeable 96 384well plates or using the novel, unique 1536well format. A lightcycler probe system consists of a pair of singlestranded fluorescentlabeled oligonucleotides. It integrates all software modules required to perform and analyze any type of experiment using the lightcycler 2. Lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils catalog no. Nov 09, 2019 lightcycler 96 is used by 2 users of software informer. Roche three detection channels, which measure fluorescence at 530, 640, and 705 nm for both onecolor and dualcolor assays. Roche lightcycler 480ii 96 pcr thermocycler 96 silber block cycler cover dell latitude i5 running windows 10 with lightcycler 480 software ver1. Roche lightcycler 96 biotecon diagnostics biotecon. The lightcycler 96 instrument is a realtime pcr system for rapid cycling up to 96 samples. A cc object can only be applied to experiments that were run on the same lightcycler 480 instrument it was created on. Used roche lightcycler roche lightcycler 480ii96 pcr.
Lightcycler 96 realtime pcr system assay for endogenous. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples. Comprehensive programming options, a large touchscreen, and intuitive analysis software make it. Realtime ready custom panels create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator. Roche lightcycler 480 ii roche lightcycler 96 dualo 32 dualo 32 r 2 other laboratory equipment foodproof dlight oculyze microproof software suite microproof diagnostic interpreter microproof lirix user cockpit microproof nucleic acid normalization tool microproof pcr setup wizard technical service consumables industries beverages beer fish and.
Conforms to the european directive for in vitro diagnostic medical devices 9879ec in combination with the lightcycler 2. Applications include absolute and relative quantification, melting curve analysis, and. Roche lightcycler 480 ii 96 thermocycler for sale online. It offers fast and accurate analysis that impresses with its intuitive and userfriendly software. Roche today announced the global launch of new software updates, including advanced high resolution melting analysis and qualitative gene detection modules for the light cycler 96 and new support features for the lightcycler nano system. Intuitive touchscreen and powerful analysis modules.
The innovative lightcycler 96 application and instrument software creates a simple analysis workflow with easy import and export functions, email notifications. Roche lightcycler 480 real time rt pcr qpcr thermal. Lightcycler software roche, free lightcycler software roche software downloads, page 3. Used roche lightcycler roche lightcycler 480ii96 pcr unit 3. Based on a comprehensive improvement of the peltierbased technology incorporated in a silver block, the instrument provides extraordinary welltowell temperature homogeneity and maximized interwell, intercycle reproducibility. New software releases and user guides for the lightcycler 96 instrument are avail able in the download area of the roche applied sciences website. The lightcycler 96 is a realtime pcr cycler for medium sample throughput. The most popular version of this product among our users is 1.
The lightcycler was licensed to roche in 1997, and was unveiled to the world in 1998. Roche lightcycler 480 real time rt pcr qpcr thermal cycler. Roche lightcycler 480 ii 96 thermocycler for sale online ebay. Lightcycler 96 realtime pcr system from roche life science duration. System component 2 lightcycler capillaries 96 capillaries and stoppersbox1 32 lightcycler centrifuge adapters in an aluminum cooling block1 1 lightcycler operators manual 1 lightcycler software package1 cable to connect lightcycler instrument to the computer power cord german power cord u. Please return again later or contact us if you have an urgent enquiry. Comprehensive programming options, a large touchscreen, and intuitive analysis software that is accessible to any user make it easy to obtain publication. Roche lightcycler 480ii384 pcr w thermocycler 96 s 2. Roche launches new software updates for its lightcycler 96 and lightcycler nano pcr systems. Aug 23, 20 for todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected. Supported by a broad range of software tools, realtime pcr based analysis can be performed in 32 capillaries, interchangeable 96384well plates or using the novel, unique 1536well format. The video shows a full exterior view of the roche lightcycler 480ii96 pcr w thermocycler 96s instrument, software, self test checklist and tray holder operation. For todays academic researcher labs, the new roche lightcycler. I started my high resolution melting experiments on quant studio 3 from applied biosystems but due to failure of the cycler i need to start with lightcycler 96 from roche.
Warranty information on warranty conditions are specified in the sales contract. The software is compatible with the regulations for electronic records subpart b. Accurate results expected from a lightcycler system even with a gradient. Roche lightcycler 480ii96 pcr thermocycler 96silber block cycler cover dell latitude i5 running windows 10 with lightcycler 480 software ver1. Precise lightcycler 96 instrument thermal homogeneity and cycling speed produce accurate and reproducible results in a very short time. Over 20,000 lightcyclers have been sold, providing a rapid option for realtime pcr in 1530 min. Oligo probe 1 is labeled at the 3 end with a donor reporter and oligo probe 2 is labeled at its 5 end with one of a few available acceptor reporters. Discover this compact and smart new member of the roche. Lightcycler 96 realtime pcr system super capabilities are. For todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. Lightcycler 96 system from roche applied science a member. Introducing the lightcycler 96 realtime pcr system what if you had the power to get your qpcr data fast, reliably and with less effort. Je vhodny pro stredne velke laboratore, ma moderni, velmi intuitivni software a nabizi vyjimecnou presnost, homogenitu a reporoducibilitu. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user.
Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping. Make sure the correct thermal block cycler 96well for f. Opening new horizons in the field of data analysis. The video shows a full exterior view of the roche lightcycler 480ii 96 pcr w thermocycler 96 s instrument, software, self test checklist and tray holder operation. Roche diagnostics 04729692001 lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils catalog no. Additionally, the equipment and software installations, including application. The result of more than a decade of roche experience developing highperformance qpcr systems is now accessible for any research lab. The only difference between this updated version and lightcycler software 4. Lightcycler 96 system 5 lightcycler 96 system software as smart and intuitive as you want it to be whether you are a qpcr novice or a seasoned expert, the lightcycler 96 software can accommodate your needswithout wasting your time learning a new software package. Roche lightcycler 480ii384 pcr w thermocycler 96s 2. The first fda approved kits for genetic variants factor v leiden and prothrombin appeared on the lightcycler in 2003, and a 6color version the lc 2.
Connection via network or usb stick, remote data analysis via email. Contact your roche representative for further information. Ideal for all levels of experience, the lightcycler 96 instrument provides an optimal combination of temperature homogeneity and data reproducibility. Jan 31, 20 lightcycler 96 realtime pcr system from roche life science duration.
Roche life science realtime ready overview lightcycler. The versatile, platebased lightcycler 96 system helps you achieve fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and snp analysis compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis. New innovative lightcycler 96 application and instrument software creates a simple analysis workflow with easy import and export functions, email notifications. Lightcycler 96 is used by 2 users of software informer. For todays academic research labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. The lightcycler 96 instrument provides an ideal combination of temperature homogeneity, data reproducibility, and intuitive analysis software the lightcycler 480 instrument supports mediumtohigh throughput with 96 and 384 well block options. Later, the lightcycler 480 software automatically uses this cc object to reassign the fluorescence in each channel to the appropriate dye the net result is detection of only one dye signal in each channel. Discover this compact and smart new member of the roche lightcycler realtime pcr system. The lightcycler 480 instrument ii is a rapid, platebased, thermal block cycler with integrated realtime, online detection capabilities. Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96 or 384. Comprehensive programming options, a large touchscreen, and intuitive analysis software make it easy to obtain publicationready results. Roche launches new software updates for its lightcycler 96. Used roche lightcycler 480ii96 roche lightcycler 480ii96. It is an optical detection system that allows flexible detection of sequencedependent probes, such as hydrolysis probes and sequenceindependent dyes i.
Used roche lightcycler 480ii96 roche lightcycler 480ii. Roche diagnostics lightcycler 480 plate 96 white lightcycler. Work economically, adapting workflows to your format and throughput needs. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is. Roche molecular biochemicals lightcycler operators manual. The lightcycler 96 system delivers data that makes you a.
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